Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 17;11:336. doi: 10.1038/s41467-019-13654-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 10 — a Immunocytochemical analysis of MIA PaCa-2 cells 4 days after infection with Lenti-STAND-Y13-259, Lenti-Myc-Y13-259, and Lenti-STAND-A36 (green; anti-Flag to detect scFv; blue, Hoechst staining to detect nuclei). Arrowheads indicate STAND-Y13-259 and STAND-A36 stably expressed in the cytoplasm. Arrows indicate Myc-Y13-259 aggregates. b Intracellular interaction of endogenous Kras and STAND-Y13-259 in MIA PaCa-2 cells infected with lentiviral vectors. Immunoprecipitates with anti-HA antibody were analysed using western blotting (WB) with indicated antibodies. Expression of STAND-A36 was weak compared to that of STAND-Y13-259 (arrows in middle and bottom panels). c Viability of MIA PaCa-2 cells infected with indicated lentiviral vectors, as determined using an MTS assay after 4 days (n = 3 independent samples). Statistical analysis: two-tailed one-way analysis of variance, F (3, 8) = 490.4608, P < 0.00001, Tukey’s multiple comparison test, STAND-Y13-259 vs. Myc-Y13-25, P < 0.00001; STAND-Y13-259 vs. STAND-A36, P < 0.00001; STAND-Y13-259 vs. phosphate-buffered saline (PBS), P < 0.00001; Myc-Y13-259 vs. PBS, P < 0.00001; STAND-A36 vs. PBS, P = 0.0641. d Western blotting using a tumour of a subcutaneous heterotopic xenograft model of pancreatic cancer. Pre-established tumours were injected once weekly for 4 weeks at 2 sites with lentiviral vectors. Western blot analysis of STAND-Y13-259 (upper panel) and actin (lower panel) expression in tumours dissected from mice 3 days after the fourth lentiviral vector injection. e Immunohistochemistry of STAND-Y13-259 (upper panel), STAND-A36 (middle), and Myc-Y13-259 (lower panel) in the tumour described in d. f Tumour growth as described in d. Arrows indicate the day of lentivirus injection. Statistical analysis: Kruskal–Wallis test, P = 0.0005310, Dann’s post-hoc multiple comparisons test (STAND-Y13-259: n = 6, Myc-Y13-259: n = 5, STAND-A36: n = 7, STAND-Y13-259 vs. Myc-Y13-259, P = 0.0052; Myc-Y13-259 vs. STAND-A36, P > 0.9999; STAND-Y13-259 vs. STAND-A36, P = 0.0269). g Representative digital photographs of tumour xenografts dissected from mice 24 days after the first intratumoural injection with indicated lentiviral vectors. Scale bars, 25 μm a, 100 μm e and 10 mm g. Error bars represent standard error of the mean. Source data are provided as a Source Data file.