Table 2.
Effect of three anti-cancer agents on the pools of four dNTPs in HCT116 cells.
| HCT116 | ||||
|---|---|---|---|---|
| Treatment | pmol/106 | |||
| control | Dox(1 μM) | GEM (1 μM) | 5-FdU (2 μM) | |
| — | 1 hr | 8 hr | 6 hr | |
| Recovery | — | 8 hr | — | — |
| dATP | 2.6 ± 0.2 | 3.9 ± 0.8 | Non detectable ↓(0) | 1.9 ± 0.6 |
| dTTP | 7.6 ± 3.4 | 8.1 ± 2.1 | 14.1 ± 0.9 ↑ (1.9) | Non detectable ↓(0) |
| dCTP | 1.2 ± 0.9 | 2.0 ± 1.6 | 0.9 ± 0.6 | Non detectable ↓(0) |
| dGTP | 7.1 ± 0.8 | 5.8 ± 1.9 | 1.8 ± 1.1 ↓ (0.3) | 3.2 ± 0.3 ↓ (0.45) |
Each data point represents the mean ± SD determined from two independent experiments in duplicate. Numbers in parentheses indicate fold changes relative to the control. ↑ and ↓ indicate the significant increase and decrease relative to the control. Dox, doxorubicin. GEM, gemcitabine. 5-FdU, 5-fluorodeoxyuridine