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. 2020 Jan 17;10:549. doi: 10.1038/s41598-019-57362-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protocol for experiments in which B21 was stimulated using random interstimulus intervals (ISIs) and changes in [Ca²⁺]_i and PSP induction were monitored simultaneously. (A) Sample traces from a typical experiment. The bottom trace is a current clamp recording from the soma of B21. The middle trace plots changes in calcium fluorescence quantified as percent relative change (F − F₀)/F₀. The top trace is a current clamp recording from B8. In all three traces the red arrows indicate how measurements were made. (B) Histogram plotting the ISIs for all stimuli delivered in this study.