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. 2020 Jan 17;11:338. doi: 10.1038/s41467-019-14219-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a DU-145 cells seeded at 5 × 10⁴ cells/ml in androgen-depleted medium were left untreated or treated with 5 μM GO-203 for the indicated times. Cell number (mean±SD of three determinations) was determined by trypan blue staining. b Lysates obtained on day 3 were immunoblotted with antibodies against the indicated proteins. c Castrated 6-week old nude male mice were injected subcutaneously in the flank with 3 × 10⁶ DU-145 cells. Mice were pair-matched into two groups when tumors reached 100–150 mm³ and were treated IV with vehicle or GO-203/NPs weekly for 3 weeks. Tumor volumes are expressed as the mean±SD for six mice. d Lysates from tumors obtained on day 25 were immunoblotted with antibodies against the indicated proteins. e Lysates from DU-145 and NCI-H660 cells were immunoblotted with antibodies against the indicated proteins. f NCI-H660 cells seeded at 1 × 10⁶ cells/ml in androgen-depleted medium were left untreated or treated with 5 μM GO-203 for the indicated times. Cell number (mean±SD of three determinations) was determined by trypan blue staining. g Lysates obtained on day 2 were immunoblotted with antibodies against the indicated proteins. Source data are provided as a Source Data file.