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. 2020 Jan 17;10:572. doi: 10.1038/s41598-019-57348-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) MPO activity (U/mg protein). (B) Macrophage infiltration in the colon of DSS-treated mice. Representative immunofluorescence sections of colonic macrophages by F4/80 marker. Bar represents 50 µm. (C) The average of the mean fluorescence intensity (MFI) determined by flow cytometry showing the expression of TNF bound to membrane present in macrophages isolated from colon tissue. The values presented correspond to one experiment of two with similar results. (D,E) Flow cytometry analysis of lymphoid populations in mesenteric lymphoid nodes. White: wild-type; grey: MCJ-deficient. Box and whisker plots of median, quartiles and range, n = 7 mice per group at a minimum. Statistical analysis: two-way ANOVA. “*” in boxes versus control, “*” versus WT DSS treated.