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. 2020 Jan 17;10:603. doi: 10.1038/s41598-019-57283-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Four plasmid constructs of chimeric multiepitope vaccines. (A,C) are the expression plasmids of 42E2 and 45F2 in pET28a, a prokaryotic bacterial system; (B,D) represent expression plasmids based on pcDNA3.1, a eukaryotic tilapia cell culture system.