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. 2020 Jan 17;10:603. doi: 10.1038/s41598-019-57283-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dot blot analysis of serum from vaccinated fish. (A) The detectable antibody from vaccinated fish serum is shown on the membrane compared with that for the mock, FKC (formalin killed cell) and pcDNA3.1 group (negative control) against recombinant protein of multiepitope vaccine themselves of FKC vaccine at 1 month. (B) The integrated density from all the dot blot results were converted to the values using the ImageJ 1.x server. Data are represented as the means ± SDs (n = 2). The different letters above the bars indicate significant differences within treatment (P < 0.05).