Fig. 2.

SBF2-AS1 competitively regulates miR-143 and RRS1 is targeted by miR-143. a the Subcellular localization of SBF2-AS1 was analyzed by online prediction website; b the subcellular localization of SBF2-AS1 was confirmed by FISH assay; c The binding sites between SBF2-AS1 and miR-143 were predicted by RNA22 website; d The binding relation between SBF2-AS1 and miR-143 was affirmed by dual luciferase reporter gene assay; e The enrichment of miR-143 on SBF2-AS1 was detected by RNA pull-down assay; f The binding sites of RRS1 and miR-143 were predicted by http://www.targetscan.org; g The binding relation between miR-143 and RRS1 was affirmed by dual luciferase reporter gene assay. * P < 0.05, The measurement data conforming to the normal distribution were expressed as mean ± standard deviation. The t-test was performed for comparisons between two groups, N = 3