Fig. 4.

Reduced SBF2-AS1 and overexpressed miR-143 prohibit the viability of BC cells. a The colony formation ability of MDA-MB-231 cells in each group was detected by colony formation assay; b Statistical results of colony formation ability of MDA-MB-231 cells in each group; c The colony formation ability of MCF-7 cells in each group was detected by colony formation assay; d Statistical results of colony formation ability of MCF-7 cells in each group; e MDA-MB-231 cell viability was detected by MTT assay; f MCF-7 cell viability was detected by MTT assay; g Protein bands of Ki-67 and CyclinD1 in MDA-MB-231 cells in Western blot analysis; h Statistical results of the protein expression of Ki-67 and CyclinD1 in MDA-MB-231 cells; i Protein bands of Ki-67 and CyclinD1 in MCF-7 cells in Western blot analysis; j Statistical results of the protein expression of Ki-67 and CyclinD1 in MCF-7 cells. The measurement data conforming to the normal distribution were expressed as mean ± standard deviation. One-way ANOVA was used for comparisons among multiple groups, and LSD-t test was used for pairwise comparisons after one-way ANOVA, N = 3, * P < 0.05 vs the si-NC group; # P < 0.05 vs the mimics NC group; & P < 0.05 vs the oe-SBF2-AS1 + mimics NC group