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. 2020 Jan 18;13:9. doi: 10.1186/s13068-020-1652-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Characterization of biomass porosity and cellulose accessibility in the transgenic lines and WT. a SEM images of raw material and biomass residue obtained from green liquor (Na₂S + Na₂CO₃) pretreatment. Scale bar is 10 μm. Allows as rough point. b Cellulose accessibility by measuring Congo red (CR) dye area. c, d Surface area and average pore diameter of biomass residues obtained from green liquor (Na₂S + Na₂CO₃) pretreatment. All data as mean ± SD of three technical replicates. Student’s t test was performed between the transgenic plants and WT as **P < 0.01