. 2020 Jan 18;17:27. doi: 10.1186/s12974-019-1678-y

Table 2.

Depletion of microglia reduces immune activation in response to ethanol. Brain slices were treated with vehicle or underwent microglial depletion with PLX3397 for 10 days, followed by ethanol (100 mM) treatment without PLX3397. Gene induction was assessed by RT-PCR after 4 days of ethanol treatment. Mean ± SEM

Category	Gene	Control	Depletion	Ethanol	EtOH + depletion
Cytokines	TNFα	100 ± 12	22 ± 4**	185 ± 16**	34 ± 5^††
	IL-1β	100 ± 2	15 ± 2**	264 ± 17**	37 ± 1^††
	IL-4	100 ± 14	239 ± 38*	34 ± 6*	139 ± 11^††
	IL-6	100 ± 14	356 ± 20**	186 ± 50*	335 ± 6^††
	IL-10	100 ± 4	151 ± 10*	217 ± 3**	224 ± 18^††
Toll-like receptors	TLR2	100 ± 0.4	56 ± 0.2**	184 ± 25**	68 ± 10^††
	TLR3	100 ± 2	55 ± 6**	235 ± 3**	101 ± 2^††
	TLR4	100 ± 3	104 ± 14	159 ± 1*	113 ± 6
	TLR5	100 ± 5	16 ± 2**	112 ± 9	30 ± 2
	TLR7	100 ± 9	23 ± 1**	195 ± 5**	49 ± 0.4^††
	TLR9	100 ± 8	24 ± 1**	189 ± 5**	48 ± 9^††
Microglia markers	Iba-1	100 ± 0.3	21 ± 1**	149 ± 5*	45 ± 3^†
	TREM2	100 ± 9	14 ± 0.5	76 ± 13*	32 ± 1^††
	CD200R	100 ± 9	33 ± 1**	78 ± 4*	50 ± 5^††
	CX3CR1	100 ± 3	12 ± 0.1**	69 ± 1*	116 ± 4
	IL-4R	100 ± 10	37 ± 4**	144 ± 20*	66 ± 9^††
	IRF8	100 ± 2	15 ± 0.1**	66 ± 3*	15 ± 1^††
Neuronal genes	mGluR2	100 ± 6	263 ± 56**	134 ± 11	158 ± 18^†
	mGluR3	100 ± 9	170 ± 11*	92 ± 6	206 ± 24
	mGluR5	100 ± 2	173 ± 23*	94 ± 21	167 ± 15^†
	mGluR8	100 ± 10	213 ± 38**	169 ± 4*	364 ± 6^††
	NR2A	100 ± 1	133 ± 0.5**	135 ± 5*	272 ± 7^††
	NR2B	100 ± 5	137 ± 1*	123 ± 7	264 ± 2^††
	PSD-95	100 ± 2	153 ± 21*	124 ± 1	201 ± 58^†
	BDNF	100 ± 9	115 ± 6	205 ± 8**	550 ± 4^††
	NGF	100 ± 3	115 ± 7	148 ± 21	162 ± 5*
	NGFR	100 ± 3	52 ± 4*	76 ± 8	46 ± 0.2^††
	CamK2a	100 ± 5	61 ± 4*	85 ± 3	104 ± 8
	CamK2d	100 ± 9	137 ± 2*	117 ± 11	116 ± 10
Interferons	IFNα	100 ± 5	153 ± 5*	200 ± 23**	302 ± 1^††
Interferons	IFNϒ	100 ± 9	160 ± 8**	39 ± 1**	94 ± 3

*p < 0.05, **p < 0.01 vs control; †p < 0.05, ††p < 0.01 vs ethanol