Fig. 2.
Mechanism of Ect2 inhibition. (A) Relative GEF activity of Ect2 with WT interface (WT) and various mutants. (A, Right) Schematic of the Ect2 constructs. Error bars indicate SD (n = 3 independent experiments). (B) Microscale thermophoresis (MST) analyses of Ect2 binding to RhoA. To enhance the binding, the assays were done with RhoA in the apo form in the presence of EDTA. Dissociation constants: Kd (DP) = 0.6 ± 0.2 μM; Kd (ΔB2) = 2.5 ± 0.6 μM. Error bars indicate SD (n = 3 independent experiments).