Fig. 3.

Degradation of U₃₆ and C₃₆. (A) Representative degradation reaction of U₃₆ (1 nM; Rrp6p, 110 nM) and C₃₆ (1 nM; Rrp6p, 110 nM). (B) Kinetic parameters for the degradation of U₃₆ (solid circles) and C₃₆ (open circles) for individual nucleotides (Top, degradation rate constant (k_f); Middle, apparent dissociation constant for productive binding (K_1/2^P); Bottom, ratio between apparent productive and nonproductive dissociation constants (P/NP)). Data show results of the global data fit (SI Appendix, Fig. S4). Error bars mark the SEs of the global fit. The corresponding data for A₃₆ (Fig. 2D) are shown for comparison.