Fig. 3.
The aSlo1 channel is insensitive to PAX. (A–D) Macroscopic currents of aSlo1 WT (A), F300G (B), I299G (C), and I299GF300G (D) evoked by +180-mV steps from a holding potential of 0 mV before application (black), in the presence (red), and washout (blue) of 200 nM PAX. Test pulse was applied every 5-s. [Ca2+]in was 10 μM for all of the experiments shown in this figure. (E) Fractional unblock of aSlo1 and mSlo1 currents in the presence of 200 nM PAX at 0 mV with 10 μM [Ca2+]in. The number of experiments performed on each BK construct is in parentheses. The open circles represent the individual data points of aSlo1 inhibition by 200 nM PAX. ***P = 0.0001, 1-way ANOVA with the Bonferroni post hoc test.