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. 2019 Dec 26;117(2):993–999. doi: 10.1073/pnas.1913712117

Fig. 3.

Fig. 3.

Cytoplasmic and mitochondrial isoforms of NOC are regulated by alternative translation initiation sites. (A) Cartoon representation of the coding sequences of WT and mutant NOC (mouse numbering). (B) Live-cell imaging of HeLa cells overexpressing mNOC-mCherry (shown in red) with either the YFP-MITO or the YFP-KDEL marker (shown in green). Colocalization can be seen with both markers. The rightmost column is the zoomed-in image of the area within the dotted white square in the merged column. The scale bars are 20 and 5 μM for the nonzoomed and the zoomed-in images, respectively. (C) Live-cell imaging of HeLa cells overexpressing mNOC-M1A-mCherry with either the YFP-MITO or the YFP-KDEL marker. The short isoform of NOC colocalizes with the YFP-KDEL but not the YFP-MITO marker. (D) Live-cell imaging of HeLa cells overexpressing mNOC-M65A-mCherry with either the YFP-MITO or the YFP-KDEL marker. The long isoform of NOC colocalizes with the YFP-MITO but not the YFP-KDEL marker. White arrows point to YFP-KDEL-negative membranous structures.