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. 2019 Dec 30;117(2):1119–1128. doi: 10.1073/pnas.1904022116

Fig. 6.

Fig. 6.

Intratumoral adjuvanted seasonal influenza vaccine administration fails to increase T cells but maintains regulatory B cells in the tumor. (A) Experimental design. Unadjuvanted seasonal influenza vaccine (FluVx): FluVx2. n = 3 to 5 pooled tumors/group. Data are representative of at least 2 independent experiments with similar results. (B) Cumulative pie charts of dendritic cells (CD11c+) among intratumoral APCs (CD45+MHC-II+) from experiment described in A and Fig. 4D. (C) Cumulative pie charts of CD8+ T cells (CD8+) among intratumoral T cells (CD45+CD3+) from experiment described in A and Fig. 4D. (D) Cumulative flow cytometry plots of tumor antigen-specific (gp100 dextramer+) CD8+ T cells among intratumoral CD8+ T cells (CD45+CD3+CD8+) from a similar experiment as described in A. Cumulative pie charts of B cell (CD19+) to T cell (CD3+) ratio among intratumoral T and B cells (CD45+CD3+CD19− and CD45+CD19+CD3−) from experiment described in A. (F) Cumulative pie charts showing ratio of Bregs (IL-10+) among intratumoral B cells (CD45+CD20+) from experiment described in A. (G) Cumulative pie charts of Tregs (FOXP3+) among intratumoral CD4+ T cells (CD45+CD3+CD4+) from experiment described in A. (H) Experimental design. n = 3 to 4 mice/group. Data are representative of at least 2 independent experiments with similar results. (I) Tumor growth curves from experiment described in H. (J) Experimental design. n = 4 to 10 mice/group from 2 experiments with similar results. (K) Tumor growth curves from experiment described in J. *P < 0.05, **P < 0.01, ***P < 0.001 [2-way ANOVA with Tukey correction (I and K)]. Error bars: mean ± SEM. i.d., intradermal; i.t., intratumoral; FluVx, unadjuvanted seasonal influenza vaccine; AdjFluVx, adjuvanted seasonal influenza vaccine.