Figure 4.

Nrf2 expression in MDA-MB231 treated with AEs/PTX. (a) Real-time assay. Nrf2 RNA expression is detected in cells treated with indicated concentrations of AEs plus and minus PTX (20 nM). 12.5 μM AEs vs. 12.5 μM AEs+PTX ^∗∗p = 0.0023. 25 μM AEs vs. 25 μM AEs+PTX ^∗p = 0.0176. (b) Nrf2 protein expression: total protein expression and nuclear translocation of Nrf2 were detected in treated cells. Quantification of band intensities was performed using ImageJ software, normalized by β-actin (total Nrf2) and histone H3 (nuclear Nrf2) expression levels. Relative values are calculated by comparing sample band intensities to control.