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. 2020 Jan 14;12:277–287. doi: 10.2147/CMAR.S220351

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© 2020 Chen et al.

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CASC9 interacted with miR-423-5p/SOX12. (A) A CASC9-mut as well as a CASC9-wt luciferase reporter plasmid was cloned by mutating the predicted miR-423-5p binding site in CASC9. (B) The luciferase reporter plasmids were co-transfected into 293T cells with miR-423-5p mimic or NC. The luciferase activities were measured by dual luciferase assays. (C) The miR-423-5p expression in CAL27 and Tca8113 cells transfected with siCASC9 or siCtrl was assessed by RT-qPCR. (D) Pearson’s analysis was performed to identify the correlation between miR-423-5p expression and CASC9 expression in TSCC clinical samples. (E) A SOX12-mut as well as a SOX12-wt luciferase reporter plasmid was cloned by mutating the predicted miR-423-5p binding site in SOX12. (F) The luciferase reporter plasmids were co-transfected into 293T cells with miR-423-5p mimic or NC. The luciferase activities were measured by dual luciferase assays. (G) The SOX12 expression in CAL27 and Tca8113 cells transfected with miR-423-5p mimic or NC was assessed by RT-qPCR. (H) Pearson’s analysis was performed to identify the correlation between SOX12 expression and CASC9 expression in TSCC clinical samples. Data is from three independent experiments and expressed as mean ± SD. ***P<0.001 compared with siCtrl.