Figure 4.

Immune-boosting effect of various RA/CTS polyplexes. Splenic DCs were treated with various formulations of RA/CTS polyplexes. After 24 h, the surface expression of CD80 on DCs were measured (A) and quantified (B) by flow cytometry (*** p < 0.005). (C) Naïve C57BL/6 mice were vaccinated 3 times at 4-day intervals with RA/CTS physically mixed with OVA antigen (100 μg). Five days after the last vaccination (day 0), mice were inoculated subcutaneously with B16-OVA cells. Blood samples were collected 6 days after inoculation for serum anti-OVA IgG titration at different dilution factors. (Data shown as mean ± SEM, n = 4). *** p < 0.005.