Figure 6.

In vivo antitumor effects of RA/CTS 340K polyplexes. (A) Timeline for in vivo experiments. Naïve C57BL/6 mice were vaccinated three times at 4-day intervals (days -13, -9, -5) with a mixture of RA/CTS 340K polyplexes plus OVA antigen (100 μg). Five days after the last vaccination (day 0), mice were inoculated subcutaneously with B16-OVA cells (3 × 10⁵). (B) Tumor volumes were measured until day 33 after tumor inoculation. (C) Survival of mice was monitored until day 48. n = 5 (untreated, OVA) and n = 10 (OVA + RA, OVA + RA/CTS).