Figure 2.
EAA and EAF protect PC12 cells from H2O2-induced oxidative injury. (a) The cells were treated with EAA and EAF (50, 100, and 200 μg/mL) for 24 h and then treated with DCFH-DA (20 μM) for 30 min. The intracellular levels of ROS were visualized using fluorescent confocal microscopy. (b) Quantitative measurement of intracellular ROS generation. (c–e) Cells were treated with EAA and EA (50, 100, and 200 μg/mL) for 24 h. Vitamin C (ascorbic acid) was positive control (50 μg/mL). The expression levels of SOD2 (c), CAT (d), and GPx (e) were detected by western blot analysis in PC12 cells. All protein expression levels were quantified by normalizing to β-actin levels. All columns are means ± SD (n = 3). Means with different letters on the all-color columns were significantly different at p < 0.05 based on Duncan's test. EAA: Erigeron annuus aerial parts; EAF: E. annuus flowers.