Figure 3.
EAA and EAF protect PC12 cells from H2O2-induced oxidative apoptosis. (a) Protein expression related to the intrinsic pathway of apoptosis (Bax/Bcl-xL). (b) Protein expression related to the extrinsic pathway (cleaved- (C-) pro-caspase-8 and -3). Cells were treated with EAA and EA (50, 100, and 200 μg/mL) for 24 h. Protein expression levels were detected by western blot analysis in PC12 cells. Protein expression was quantified based on normalized β-actin levels. All columns are means ± SD (n = 3). Means with different letters on the all-color column were significantly different at p < 0.05 based on Duncan's test. EAA: Erigeron annuus aerial parts; EAF: E. annuus flowers.