Skip to main content
. 2020 Jan 16;8:e8346. doi: 10.7717/peerj.8346

Figure 1. Morphological characters of the AR2 isolate.

Figure 1

(A) Culture of isolate AR2 for 3 days on PDA, scale bar = 1 cm. (B) The morphological characteristics (straight-angle branching and hyphae constriction at branching points) of mycelia of AR2 under optical microscope, scale bar = 5 μm. (C) The morphological characteristics (hyphae fusion) of mycelia of AR2 under optical microscope, scale bar = 5 μm. (D) 4′,6-diamidino-2-phenylindole (DAPI)-stained hyphae of AR2 showing binucleate cells; the red arrow represents a binucleate in a mycelial cell; bar = 100 μm. Morphological observations of mycelium were performed using the fluorescence microscope, Zeiss AxioImagerA1 (Zeiss, Germany). (E) Neighbor-joining phylogenetic tree of ITS rDNA sequences from AR2 and 18 Ceratobasidium sequences of known species from GenBank. Bootstrap values (calculated from 1,000 resamplings) of above 70% are shown at the branch point. Waitea circinata is used as the outgroup.