Figure 6.

Transcription factor MYBL2 targets LOXL1‐AS1 promoter region. A, qRT‐PCR was conducted to detect the relative expression of LOXL1‐AS1 in sh‐MYBL2 transfected LUAD cells. B, Luciferase activity of LOXL1‐AS1 promoter upon MYBL2 knockdown was determined. C, The luciferase activity of P1, P2, P3, and P4 in LOXL1‐AS1 promoter region was evaluated by luciferase reporter assay. D, ChIP assay was applied to further confirm the interaction between MYBL2 and LOXL1‐AS1 promoter. E, MYBL2 was predicted to have a binding site in LOXL1‐AS1 promoter, which was subsequently validated by luciferase reporter assay. Data were compared under Student's t test. **P < .01, ***P < .001 are statistical symbols that indicated the significant difference between groups. ChIP, chromatin immunoprecipitation; LOXL1‐AS1, LOXL1 antisense RNA 1; LUAD, lung adenocarcinoma; qRT‐PCR, quantitative real‐time PCR