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. 2019 Dec 4;9(2):640–652. doi: 10.1002/cam4.2733

Figure 3.

Figure 3

IL22 induces upregulation of MUC1 expression in HNSCC and the changes of T‐cell function. A, Exogenous addition of human IL22 recombinant protein could induce upregulation of MUC1 expression in HNSCC cell lines except OME and Cal27, which is highest at 72 h. The red is negative control; the blue is MUC1 expression; the orange is MUC1 expression after addition IL22 recombinant protein for 72 h. B, The left panel is normal CD3+ T‐cell differentiation; the middle panel is the characterization of CD3+ T‐cell differentiation by exogenous addition the IL22 recombinant protein for 72 h (Naïve: CD45RA+/CD62L+; Central Memory: CD45RA−/CD62L+; Effector Memory: CD45RA−/CD62L−; Effective: CD45RA+/CD62L−). The right panel is the statistical table. C, After 72 h of exogenous addition of IL22 recombinant protein, detected the killing of CAR‐MUC1 T cells against OME cell line and different HNSCC cell lines in different E/T ratios. The results were the sum of Annexin V single‐positive rate (early apoptosis) and PI, Annexin V double‐positive rate (late apoptosis). D, After 72 hours of exogenous addition of IL22 recombinant protein, cytokine secretion in coculture supernatants after different E/T ratio were measured by ELISA assay. (Error bars represent the mean ± SEM. **P < .01; ns, not significant)