Figure 2.

LncRNA LUCAT1 knockdown inhibits proliferation and migration of PDAC cells. (A) PANC‐1 cells and AsPC‐1 cells were transfected with sh‐NC, sh‐lncRNA#1 or sh‐lncRNA#2, followed by qRT‐PCR analysis to assess transfection efficiency. (B) MTT assays were performed to measure proliferation of PANC‐1 cells and AsPC‐1 cells following transfection with sh‐NC or sh‐lncRNA#1. (C) Flow cytometry was carried out to evaluate cell cycle of PANC‐1 cells and AsPC‐1 cells following transfection with sh‐NC or sh‐lncRNA#1. (D) Migration capability and (E) invasion capability of AsPC‐1 and PANC‐1 cells were examined by Transwell migration assays and Transwell invasion assays, respectively. **P < .01. lncRNA LUCAT1, long non‐coding RNA lung cancer associated transcript 1; sh‐NC, negative control short hairpin RNA; sh‐lncRNA#1, short hairpin RNA#1 specifically targeting lncRNA LUCAT1; sh‐lncRNA#2, short hairpin RNA#2 specifically targeting lncRNA LUCAT1; qRT‐PCR, quantitative real‐time polymerase chain reaction