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. 2019 Dec 2;9(2):757–767. doi: 10.1002/cam4.2724

Figure 4.

Figure 4

LncRNA LUCAT1 is a molecular sponge of miR‐539. (A) A complementary binding site of lncRNA LUCAT1 in miR‐539 was predicted via starBase v2.0 online database. (B) RIP assays were conducted to validate whether lncRNA could interact with miR‐539 in HEK293T cells. (C) Luciferase reporter assays were carried out to verify the direct binding of lncRNA LUCAT1 and miR‐539 in HEK293T cells. (D) miR‐539 expression levels were detected by qRT‐PCR analysis after transfection with sh‐NC or sh‐lncRNA#1 in AsPC‐1 and PANC‐1 cells. (E) miR‐539 expression levels in tumorous tissues and normal cerebellar tissues were examined using qRT‐PCR analysis. (F) Spearman's correlation analysis was performed to evaluate the relationship between lncRNA LUCAT1 expression and miR‐539 expression in cancerous tissues. **P < .01. lncRNA LUCAT1, long non‐coding RNA lung cancer associated transcript 1; sh‐NC, negative control short hairpin RNA; sh‐lncRNA#1, short hairpin RNA#1 specifically targeting lncRNA LUCAT1; sh‐lncRNA#2, short hairpin RNA#2 specifically targeting lncRNA LUCAT1