Figure 5.

miR‐539 inhibitor reverses the suppressing effects of lncRNA LUCAT1 knockdown on PDAC cell proliferation and migration. (A) Proliferation, (B) cell cycle progression, (C) migration ability, and (D) invasion ability of PANC‐1 cells were determined following transfection with sh‐lncRNA#1 and miR‐539 inhibitor by MTT assays, flow cytometry, and Transwell migration and invasion assays, respectively. (E) The expression levels of cell proliferation‐related proteins (CCND1 and p21) and cell motility‐associated proteins (Snail and Vimentin) in PANC‐1 cells were examined by western blotting assays following transfection with sh‐lncRNA#1 and miR‐539 inhibitor. **P < .01. lncRNA LUCAT1, long noncoding RNA lung cancer associated transcript 1; sh‐NC, negative control short hairpin RNA; sh‐lncRNA#1, short hairpin RNA#1 specifically targeting lncRNA LUCAT1; sh‐lncRNA#2, short hairpin RNA#2 specifically targeting lncRNA LUCAT1; CCND1, cyclin D1