Figure 4.

Overexpression of MBNL1‐AS1 inhibited the proliferation and induced the apoptosis of BC cells. A, Relative expression of MBNL1‐AS1 in 5637 and T24 cells was assessed by qRT‐PCR. B, The viable cells were evaluated using MTT assay in 5637 and T24 cells. C, Flow cytometry analysis was performed to determine the cell cycle distribution of 5637 and T24 cells. D, The percentage of apoptotic cells was detected by flow cytomentry in 5637 and T24 cells. *P < .05, **P < .01, ***P < .001. BC, bladder cancer; MBNL1‐AS1, muscleblind‐like 1 antisense RNA 1; qRT‐PCR, quantitative real‐time PCR