Figure 5.

MBNL1‐AS1 was directly targeted by miR‐135a and positively regulated PHLPP2/FOXO1 expression. A, The sequences of MBNL1‐AS1 binding with miR‐135a. B, Luciferase reporter assay was carried out to evaluate the binding activity between MBNL1‐AS1 and miR‐135a. C, Relative expression of miR‐135a was determined using qRT‐PCR. D, The protein levels of PHLPP2, FOXO1, p‐AKT, and AKT were detected with western blot. *P < .05, **P < .01, ***P < .001. MBNL1‐AS1, muscleblind‐like 1 antisense RNA 1; qRT‐PCR, quantitative real‐time PCR