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The connection between miR-503 and Apelin-12. (A) starBase predicts miR-503 and Apelin binding sites. (B) HMEC-1 were transfected with luciferase plasmids of Apelin-WT or Apelin-MUT for 48 h. After that, a dual-luciferase reporter assay was performed. (C) qPCR detection of miR-503 expression level. (D) Apelin-12 level measured by qPCR and ELISA assay. All data were represented by 3 independent experiments. *P < 0.05; **P < 0.01.
