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Inhibition of Apelin-12 on oxidative stress and inflammation induced by HG in HMEC-1 cells. (A) Cell apoptosis assessed by flow cytometry. (B) Measuring of ROS generation by DCFH-DA assay. (C) The ELISA kit detects MDA and SOD levels. (D) qPCR detection of TNF-α, IL-1β, IL-6 mRNA expression levels. (E) ELISA measurement of protein expression levels of TNF-α, IL-1β and IL-6. All data were represented by 3 independent experiments. *P < 0.05; **P < 0.01.
