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. 2019 Dec 31;28:105053. doi: 10.1016/j.dib.2019.105053

Fig. 3.

Fig. 3

RNA isolation quality data and per base sequence quality of RNA-sequencing reads. (A) RNA quality of ribosome-free, monosome, polysome and total RNA from hASCs determined using the RNA 6000 Nano Chip. (B) Examples of electropherograms of ribosome-free, monosomal, polysomal and total RNA from hASCs as determined using the RNA 6000 Nano Chip. (C) Examples of per base sequence quality scores for ribosome-free, monosomal, polysomal and total RNA samples sequenced by FastQC (version 0.11.3). (D–E) MDS plot of polysomal and total RNA fractions for adipogenic (D) and osteogenic (E) differentiation. CT: control, hASCs treated with maintenance medium for 24 h; ADI: hASCs treated with adipogenic medium for 24 h; OST: hASCs treated with osteogenic medium for 24 h.