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. 2019 Dec 31;28:105053. doi: 10.1016/j.dib.2019.105053

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© 2019 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 4 — RT-qPCR validation of six differentially expressed genes. (A–B) Fold change differences in expression after osteogenic differentiation (OSTvsCT) in total and polysomal fractions comparing RT-qPCR and RNA-Seq. (C–D) Fold change differences in expression after adipogenic differentiation (ADIvsCT) in total and polysomal fraction comparing RT-qPCR and RNA-Seq. Fold change differences were calculated using log₂ base values. RT-qPCR analysis of the total fraction was performed in technical and biological triplicate. RT-qPCR analysis of the polysomal fraction was performed in technical triplicate and biological duplicate. RNA-Seq analysis was performed in biological triplicate.