Table 5.
RT-qPCR primer sequences. Oligonucleotide primers used to analyze the differential expression of genes after 24 h of adipogenic and osteogenic differentiation.
| Gene Name | Forward Primer Sequence 5′-3′ | Reverse Primer Sequence 5′-3′ | bp |
|---|---|---|---|
| ADH1B | GGTGGCTGTAGGAATCTGTCACAC | GGGTCCCCCGAGGATTGCCT | 248 |
| MT1E | CCTGCTGCCCCATGAGCTGT | CCGGACATCAGGCACAGCAGC | 90 |
| FADS2 | CAAAAGCCGAAAGCGAAGAGG | CCCACGAATTCCAGGTCAGG | 368 |
| VEGFA | CTACCTCCACCATGCCAAGTG | TGCGCTGATAGACATCCATGA | 101 |
| WNT2 | TGAACGCCCCTCTCGGTGGA | GCCCTGGCTAATGGCACGCA | 203 |
| CRABP2 | AGAGAACCTGACGACCCGGCG | TCCACTGCTGGCTTGGACGC | 232 |
| GAPDH | GGCGATGCTGGCGCTGAGTAC | TGGTTCACACCCATGACGA | 149 |