Figure 1.

HOXA5 Is Expressed At a Poor Level in Breast Cancer Tissues and Cells

(A) Volcano map of differentially expressed genes in breast cancer. The abscissa represents log10 p value, and the ordinate represents logFC. Red dots represent upregulated genes in breast cancer, whereas green dots represent downregulated genes in breast cancer. (B) GO analysis for gene-enriched items of biological process. (C) Expression of HOXA5 in breast cancer tissues and adjacent normal tissues. (D) qRT-PCR analysis of HOXA5 expression in breast cancer tissues and adjacent normal tissues (n = 122). (E and F) Representative images (400 times) and quantitative analysis of HOXA5 expression in breast cancer tissues and adjacent normal tissues detected by immunohistochemistry (n = 122). (G) qRT-PCR analysis of HOXA5 expression in breast epithelial cell line HBL-100 and breast cancer cell lines (MCF-7, MDA-MB-231, and MCF10A). Data in (D) are measurement data and expressed as the mean ± SD, and comparisons between two groups are analyzed by paired t test. Data in (F) are enumeration data and expressed as number of cases or percentage. Comparisons between two groups are analyzed by chi-square test. Data in (G) are measurement data and expressed as the mean ± standard deviation, and comparisons among multiple groups are analyzed by ANOVA with Dunnett’s post hoc test. The experiment is repeated three times. *p < 0.05 compared with adjacent normal tissues; #p < 0.05 compared with breast epithelial cell line HBL-100.