Figure 4.

CDX2 Is the Target Gene of miR-181d-5p

(A) The predicted miRNAs that regulate CDX2. The blue part represents highly expressed miRNAs in breast cancer-related gene-expression dataset GSE59247, and the green part represents the predicted results from TargetScan. (B) The binding site of miR-181d-5p and CDX2 predicted by TargetScan. (C) qRT-PCR analysis of miR-181d-5p expression in breast cancer tissues and adjacent normal tissues (n = 122). (D) The target relationship between miR-181d-5p and CDX2 verified by dual luciferase reporter assay. (E) qRT-PCR analysis of CDX2 expression in MCF-7 cells. (F and G) Western blot analysis of CDX2 expression in MCF-7 cells. The band intensity is assessed. The above data are measurement data and expressed as the mean ± SD. Comparisons between two groups are analyzed by paired t test (C) and nonpaired t test (D, E, and G). (H) Statistical histograms of protein expression determined by western blot analysis. The experiment is repeated three times. *p < 0.05 compared with the treatment of mimic-NC; #p < 0.05 compared with the treatment of inhibitor-NC.