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. 2020 Jan 9;10:3024. doi: 10.3389/fimmu.2019.03024

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Copyright © 2020 Schaffert, Hanić, Novokmet, Zaytseva, Krištić, Lux, Nitschke, Peipp, Pezer, Hennig, Rapp, Lauc and Nimmerjahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of extrinsic IgG sialylation in μMT and ST6Gal1 deficient mice. Shown are the relative changes (% of total IgG glycoforms in the preparation) in glycopeaks (GP) 25 and 26 in IVIg (A,C) or neuraminidase treated IVIg (B,D) before (IVIg or NeuIVIg) and 2, 4, and 6 days after injection into μMT (A,B) or ST6Gal1 deficient mice (C,D) by HILIC-UPLC-FLR analysis. Changes in the respective glycopeak in individual mice are shown at the top of each figure (n = 4–5). The bottom of each Figure shows a representative HILIC-UPLC-FLR trace of GP25 and 26. In each subfigure a schematic drawing of the sugar structure for GP25 and 26 is depicted. The Figure legend at the bottom explains the symbols used for the schematic representation of the sugar moieties.