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. 2020 Jan 9;10:3024. doi: 10.3389/fimmu.2019.03024

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Copyright © 2020 Schaffert, Hanić, Novokmet, Zaytseva, Krištić, Lux, Nitschke, Peipp, Pezer, Hennig, Rapp, Lauc and Nimmerjahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Discrimination of de novo sialylation of human IgG in vivo between Fab and Fc fragments. (A,B) Serum IgG preparations of μMT mice injected with neuraminidase treated IVIg (NeuIVIg) were digested to generate Fab and Fc fragments. To identify de novo generated IgG sugar structures on the individual N-glycosylation sites, individual fractions were analyzed separately by xCGE-LIF. Depicted are representative overlays of IgG Fab- (A) and Fc-portion (B) specific xCGE-LIF glycoanalysis of C57BL/6 mice, the injected NeuIVIg preparation before injection as well as NeuIVIg 4 days after injection into μMT mice. Schematic drawings of the sugar moieties are depicted for the major peaks. Enlarged insets emphasize peaks detected selectively on NeuIVIg 4 days post injection into μMT mice. The Figure legend at the bottom of the Figure depicts the symbols used for individual sugar residues. See also Figure S2 for additional peak information. MTU”, normalized migration time units.