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. 2020 Jan 9;10:1522. doi: 10.3389/fphar.2019.01522

Figure 4.

Figure 4

Effects of columbianadin (CBN) or osthole (OST) on the I–V currents of total Ica in the absence of any calcium channel blockers in small dorsal root ganglion (DRG) neurons. (A) Representative whole-cell total Ica recorded from a small DRG neuron (neuron A) in the absence of any compound. Total Ica were obtained by 200-ms depolarizing pulses from a holding potential of −100 mV to test potentials ranging from −80 to +50 mV in a 5 mV increment. The interpulse time is 3 s. (B, C) Representative Ica in the presence of 100 μM CBN or 100 μM OST from two different DRG neurons (neuron B and C, respectively). Recordings are all from independent cells and each cell is only used for a single treatment. CBN or OST were pre-treated for 10–60 min in the recording chamber and maintained during recordings. The time for pre-treating ranged between 10 and 60 min. (D) Current-voltage relationships of the total Ica in control (black line), OST (blue line), and CBN (red line) groups. Data points are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control, #p < 0.05, ##p < 0.01, vs. CBN, two-way repeated ANOVA followed by post hoc Bonferroni analysis.