Figure 1.

Cytotoxic and growth inhibitory effect of Brv-A in breast carcinoma cells. (A) MCF-7 and (B) MDA-MB-231 cells were treated with indicated concentrations of Brv-A in presence or absence of NAC (5 mM) for 24 h and cell viability was measured by CCK-8 kit. (C) MCF-7 cells were treated with indicated concentrations of Brv-A in presence or absence of NAC for 24 h and changes in cellular morphology were photographed by phase contrast microscope (Leica, DMIL LED). (D, E) MCF-7 cells were treated with Brv-A in dose-dependent manner in presence or absence of NAC. Cell death percentage was measured by live/dead assay using fluorescent probe calcein-AM and PI. (F) MCF-7 cells were treated with indicated concentrations of Brv-A in presence or absence of NAC for 24 h and 300 cells/well were seeded into six-well plate within DMEM. Cells were kept for 10 days to form colonies. After fixation with 4% paraformaldehyde, colonies were stained with crystal violet stain and photographed. (G) Stain picked by colonies was dissolved in methanol and optical density was measured at 595 nm. (C, D) Scale bar is 100 µm. (A, B, E, G) Data are expressed as Mean ± SD while all experiments were performed in triplicate independently. *p <0.05, **p <0.01, ***p <0.001 vs untreated group (control) while ^#p <0.05, ^##p <0.01, ^###p <0.001 vs 15 µM treated group.