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. 2020 Jan 15;13:435–450. doi: 10.2147/OTT.S228702

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© 2020 Saleem et al.

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Effect of Brv-A on cell cycle distribution in MCF-7 cells. MCF-7 cells were treated with indicated concentrations of Brv-A in presence or absence of NAC for 24 h, stained with PI and analyzed by flow cytometry. Representative DNA fluorescence histograms of PI-stain cells show the cell cycle distribution. Histograms show number of cells on y-axis while DNA content on x-axis. The values display percentages of cells in indicated phases of cell cycle. Data are expressed as Mean ± SD while all experiments were performed in triplicate independently. *p <0.05, **p <0.01, ***p <0.001 vs untreated group (control) while ^###p <0.001 vs 15 µM treated group.