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. 2019 Sep 26;15(1):52–62. doi: 10.1021/acschembio.8b00849

Figure 5.

Figure 5

Interactions between HC106A and DosS heme. WT DosS protein was treated with dithionite (DTN) and then 100 μM HC106A (a) or 100 μM pf CORM-2 (a CO donor) (b). The UV–visible spectra of the two treatments exhibited a shift of the Soret peak to a common position of 422 nm. (c) DosS with a G117L amino acid substitution provides resistance to HC106A. (d) Overexpression of DosS protein promotes resistance to 20 μM HC106A treatment in Mtb. hspX and tgs1 transcripts were analyzed by qRT-PCR (***P value <0.0001 based on a t-test). The error bar represents the standard derivation of the mean for three technical replicates.