Fig. 3.
MiR-7 deficiency alters the composition of immune cells and the transduction of NF-κB, ERK signaling pathways in BTI model. WT mice and miR-7def mice were intraperitoneally injected with LPS (2.5 mg/kg of body weight) and control group with PBS. After 12 h, a–h the number of astrocytes in hippocampus and microglia in cerebral cortex were analyzed by IF and IHC and calculated (n = 3, one-way ANOVA, **P < 0.01). i, j The protein levels of AKT, phos-AKT, ERK1/2, phos-ERK1/2, NF-κB, and phos- NF-κB in brain tissue were analyzed by Western blot assay and calculated, respectively (n = 3, one-way ANOVA, **P < 0.01)