Fig. 6.
Silence of RORα aggravates the pathology of miR-7defBTI mice. MiR-7def mice (n = 6 per group) were transfected with RORα RNAi or NC RNAi through lateral ventricle. After 3 days, these mice were intraperitoneally injected with LPS (2.5 mg/kg of body weight). Twelve hours later, brain tissue was collected. a The pathology was observed by H&E (n = 3 per group). Arrows in c indicate nuclear fragments. b The mRNA levels of cytokines (IL-1β, IL-6, TNF-α, and TGF-β) were analyzed by real-time PCR assay and calculated (n = 6, one-way ANOVA, **P < 0.01). c–f The protein levels of cytokines were determined by ELISA assay and calculated (n = 5, one-way ANOVA, **P < 0.01). g–n The number of astrocytes in hippocampus and microglia in cerebral cortex were analyzed by IF and IHC (n = 3, one-way ANOVA, **P < 0.01). o, p The protein levels of signaling molecules including AKT, phos-AKT, ERK1/2, phos-ERK1/2, NF-κB, and phos-NF-κB in brain tissue were analyzed by Western blot assay and calculated (n = 3, one-way ANOVA, **P < 0.01)