Table 1.
Gene | Primer sequence (5′-3′) |
---|---|
TGF-β | F: CCCCATTCCTACTTCTCC |
R: ACGCACCTTTCTGGTTACAC | |
IL-6 | F: AGACAAAGCCAGAGTCCTTCAG |
R: GGTCTTGGTCCTTAGCCACTC | |
TNF-α | F: TGTCTACTGAACTTCGGGGTG |
R: CTGCTCCTCCACTTGGTGGTT | |
IL-1β | F: GAGCTTCAGGCAGGCAGTAT |
R: TTGTTCATCTCGGAGCCTGTA | |
TROVE2 | F: GAAGTGTGTCGCATTCCGACC |
R: GGACAGTCGGAGGAGATCTTT | |
RORα | F: GGAGACAAATCGTCAGGAATC |
R: ACCAAACTTGACAGCATCTCG | |
Col4a3bp | F: TTGAAGCTGCTCTTGACAGAC |
R: TCTATGCGTCCCGACAGAAGA | |
Gpr158 | F: AATTAGAAGCAGCCCAATGG |
R: TTTCACGAACAGCACAAAGAA | |
Zfp212 | F: ACCAAGTCACCCACCATCTCT |
R: GAGAACCTGCTTCGAAACAA | |
Socs4 | F: CCCCAGTGCCTGTATGTTCTT |
R: CTGCTGCTCTGGCACATCAAT | |
C2cd2 | F: TCAGGCCTTAGCCATGTGT |
R: CGTGGGGACTTGAGTTTCA | |
Syt4 | F: GAGAAGCTGGGGACACTCTT |
R: ACGGGTCAGAGGTCATGGATT | |
Spock3 | F: CAGTCTGTGGTTCTGATGGGC |
R: GACTTATCGGAGGGACATGG | |
Gpr22 | F: GCAAAACACCAACTGCTCCAA |
R: ACTGCATGTTGATTTCCAGAA | |
Basp1 | F: CTCTTTGACGGCCACGCTTTG |
R: CTGAGCAAGAAGAAGAAGGGCT | |
Tmed5 | F: TCGATCTCCAACGATGCCTT |
R: TCACACCCTCTTTGGACAGTG | |
Ptprj | F: TGCCATTTGCATTGCTCCAG |
R: CTTAAGCCCAGGCACTTCGT | |
Zfp606 | F: TCATGGACCAGTCTTGGGGG |
R: ATCTCACCTCGACTTGGGCTA | |
Aqp11 | F: TGCAGGAATCCCATCCACAC |
R: CCCTCCTGCATAGGCCAAAA | |
1110059G10Rik | F: TCTTCAGCTGTTAGGTCTCCC |
R: GAGCAAGCGGAACCAAGTGT | |
Gtdc1 | F: CACCCTTCTGTGTGGAGCTGA |
R: TTACGGGGTAAGTAGCCCCA | |
GAPDH | F: GAAGGTCGGAGTCAACGGATT |
R: ATGGGTGGAATCATATTGGAA | |
miR-7 | F: CGGCGGTGGAAGACTAGTGATT |
U6 | F: AGAGAAGATTAGCATGGCCCCTG |
Common reverse | R: ATCCAGTGCAGGGTCCGAGG |
Note: TROVE2 TROVE domain family, member 2; RORα RAR-related orphan receptor alpha; Col4a3bp collagen, type IV, alpha 3 (Goodpasture antigen) binding protein; Gpr158 G protein-coupled receptor 158; Zfp212 Zinc finger protein 212; Socs4 suppressor of cytokine signaling 4; C2cd2 C2 calcium-dependent domain containing 2; Syt4 synaptotagmin IV; Spock3 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3; Gpr22 G protein-coupled receptor 22; Basp1 brain abundant, membrane attached signal protein 1; Tmed5 transmembrane emp24 protein transport domain containing 5; Ptprj protein tyrosine phosphatase, receptor type, J; Zfp606 zinc finger protein 606; 1110059G10Rik RIKEN cDNA 1110059G10 gene; Gtdc1 glycosyltransferase-like domain containing 1; F forward primer; R Reverse primer