Skip to main content
. 2020 Jan 20;17:28. doi: 10.1186/s12974-020-1710-2

Table 1.

The primer sequence used for real-time quantitative PCR

Gene Primer sequence (5′-3′)
TGF-β F: CCCCATTCCTACTTCTCC
R: ACGCACCTTTCTGGTTACAC
IL-6 F: AGACAAAGCCAGAGTCCTTCAG
R: GGTCTTGGTCCTTAGCCACTC
TNF-α F: TGTCTACTGAACTTCGGGGTG
R: CTGCTCCTCCACTTGGTGGTT
IL-1β F: GAGCTTCAGGCAGGCAGTAT
R: TTGTTCATCTCGGAGCCTGTA
TROVE2 F: GAAGTGTGTCGCATTCCGACC
R: GGACAGTCGGAGGAGATCTTT
RORα F: GGAGACAAATCGTCAGGAATC
R: ACCAAACTTGACAGCATCTCG
Col4a3bp F: TTGAAGCTGCTCTTGACAGAC
R: TCTATGCGTCCCGACAGAAGA
Gpr158 F: AATTAGAAGCAGCCCAATGG
R: TTTCACGAACAGCACAAAGAA
Zfp212 F: ACCAAGTCACCCACCATCTCT
R: GAGAACCTGCTTCGAAACAA
Socs4 F: CCCCAGTGCCTGTATGTTCTT
R: CTGCTGCTCTGGCACATCAAT
C2cd2 F: TCAGGCCTTAGCCATGTGT
R: CGTGGGGACTTGAGTTTCA
Syt4 F: GAGAAGCTGGGGACACTCTT
R: ACGGGTCAGAGGTCATGGATT
Spock3 F: CAGTCTGTGGTTCTGATGGGC
R: GACTTATCGGAGGGACATGG
Gpr22 F: GCAAAACACCAACTGCTCCAA
R: ACTGCATGTTGATTTCCAGAA
Basp1 F: CTCTTTGACGGCCACGCTTTG
R: CTGAGCAAGAAGAAGAAGGGCT
Tmed5 F: TCGATCTCCAACGATGCCTT
R: TCACACCCTCTTTGGACAGTG
Ptprj F: TGCCATTTGCATTGCTCCAG
R: CTTAAGCCCAGGCACTTCGT
Zfp606 F: TCATGGACCAGTCTTGGGGG
R: ATCTCACCTCGACTTGGGCTA
Aqp11 F: TGCAGGAATCCCATCCACAC
R: CCCTCCTGCATAGGCCAAAA
1110059G10Rik F: TCTTCAGCTGTTAGGTCTCCC
R: GAGCAAGCGGAACCAAGTGT
Gtdc1 F: CACCCTTCTGTGTGGAGCTGA
R: TTACGGGGTAAGTAGCCCCA
GAPDH F: GAAGGTCGGAGTCAACGGATT
R: ATGGGTGGAATCATATTGGAA
miR-7 F: CGGCGGTGGAAGACTAGTGATT
U6 F: AGAGAAGATTAGCATGGCCCCTG
Common reverse R: ATCCAGTGCAGGGTCCGAGG

Note: TROVE2 TROVE domain family, member 2; RORα RAR-related orphan receptor alpha; Col4a3bp collagen, type IV, alpha 3 (Goodpasture antigen) binding protein; Gpr158 G protein-coupled receptor 158; Zfp212 Zinc finger protein 212; Socs4 suppressor of cytokine signaling 4; C2cd2 C2 calcium-dependent domain containing 2; Syt4 synaptotagmin IV; Spock3 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3; Gpr22 G protein-coupled receptor 22; Basp1 brain abundant, membrane attached signal protein 1; Tmed5 transmembrane emp24 protein transport domain containing 5; Ptprj protein tyrosine phosphatase, receptor type, J; Zfp606 zinc finger protein 606; 1110059G10Rik RIKEN cDNA 1110059G10 gene; Gtdc1 glycosyltransferase-like domain containing 1; F forward primer; R Reverse primer