Figure 4.

Reconstitution of EST to the liver abolishes the pulmonoprotective effect of Est ablation. (A) Schematic representation of the creation of the KOLE transgenic mice. (B) Expression of EST in the liver and lung as measured by western blotting. (C-H) Female Est^-/- and KOLE mice were subjected to the sham surgery or HS/R. Mice were sacrificed 4 hours after the initiation of HS. Shown are H&E staining of the lung sections (C, original magnification 200x). The section shows interstitial edema and infiltrated blood cells in the lung tissue (arrowhead). BALF protein concentrations (D), lung W/D ratio (E), serum estradiol levels (F), lung estradiol levels (G), and serum estradiol sulfate levels (H) were also shown. Data of estradiol and estradiol sulfate levels in Est^-/- mice are from the same mice in Fig. 3. Results are presented as mean ± SEM, (n = 4–5 for each group). *P < 0.05; **P < 0.01, with the comparisons labeled.

Abbreviations: BALF, bronchoalveolar lavage fluid; H&E, hematoxylin and eosin; HS, hemorrhagic shock; R, resuscitation; SEM, standard error of the mean; W/D, wet/dry.