Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Ident-ifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) |
Eco BL21(DE3) | EMD Millipore (Burlington, MA) | ||
| Recombinant DNA reagent | pACYCDuet-1_Ec_rpoZ | PMID: 21416542 | ||
| Recombinant DNA reagent | pEcrpoABC(-XH)Z | PMID: 21416542 | ||
| Recombinant DNA reagent | pET28a | EMD Millipore | ||
| Recombinant DNA reagent | pET28a-His10-SUMO rpoD | PMID: 28988932 | ||
| Recombinant DNA reagent | pET28a-His10-SUMO traR (pRLG15142) | This paper | Encodes Eco TraR with N-terminal His10-SUMO tag (Darst lab) | |
| Recombinant DNA reagent | pRLG770 | PMID: 2209559 | In vitro transcription vector, AmpR | |
| Recombinant DNA reagent | pRLG770-rrnB P1 (pRLG13065) | PMID: 27237053 | rrnB P1 with −88 to +50 endpoints | |
| Recombinant DNA reagent | pRLG770-argI (pRLG13098) | PMID: 11162084 | pargI with −45 to +32 endpoints | |
| Recombinant DNA reagent | pRLG770-hisG (pRLG13099) | PMID: 15899978 | phisG with −60 to +1 endpoints | |
| Recombinant DNA reagent | pRLG770-rpsT P2 (pRLG14658) | PMID: 21402902 | rpsT P2 with −89 to +50 endpoints | |
| Recombinant DNA reagent | pRLG770-thrABC (pRLG15276) | PMID: 11162084 | pthrABC with −72 to +16 endpoints | |
| Recombinant DNA reagent | pT7 αββ'(Δ943–1130) (pIA331) | PMID: 12511572 | ∆Si3 RNAP | |
| Recombinant DNA reagent | pIA900 rpoB Δ225–343ΩGG (pRLG12586) | PMID: 28652326 | ∆Si1 RNAP | |
| Recombinant DNA reagent | pET28a-His10-SUMO P43A traR (pRLG14844) | This paper | Encodes Eco TraR[P43A] with N-terminal His10-SUMO tag (Gourse lab) | |
| Recombinant DNA reagent | pET28a- His10- SUMO P45A traR (pRLG14846) | This paper | Encodes Eco TraR[P45A] with N-terminal His10-SUMO tag (Gourse lab) | |
| Recombinant DNA reagent | pET28a-His10- SUMO E46A traR (pRLG14847) |
This paper | Encodes Eco TraR[E46A] with N-terminal His10-SUMO tag (Gourse lab) | |
| Recombinant DNA reagent | pET28a-His10-SUMO R49A traR (pRLG15278) | This paper | Encodes Eco TraR[R49A] with N-terminal His10-SUMO tag (Gourse lab) | |
| Recombinant DNA reagent | pET28a-His10-SUMO K50A traR (pRLG15279) | This paper | Encodes Eco TraR[K50A] with N-terminal His10-SUMO tag (Gourse lab) | |
| Sequence-based reagent | P43A traR | IDT, this paper | 5’ GAAGCATGCGGAAATGCTATTCCGGAAGCC 3’ (Gourse lab) | |
| Sequence-based reagent | P45A traR | IDT, this paper | 5’ GGAAATCCTATTGCGGAAGCCCGGCGG 3’ (Gourse lab) | |
| Sequence-based reagent | E46A traR | IDT, this paper | 5’ GGAAATCCTATTCCGGCAGCCCGGCGGAAAATA 3’ (Gourse lab) | |
| Sequence-based reagent | R49A traR | IDT, this paper | 5’ ATTCCGGAAGCCCGGGCGAAAATATTTCCCGGT 3’ (Gourse lab) | |
| Sequence-based reagent | K50A traR | IDT, this paper | 5’ ATTCCGGAAGCCCGGCGGGCAATATTTCCCGGT 3’ (Gourse lab) | |
| Sequence-based reagent | SumoF | IDT, this paper | 5’ GGGGAATTGTGAGCGGATAACAATTCC 3’ (Gourse lab) | |
| Sequence-based reagent | SumoR | IDT, this paper | 5’ GTCCCATTCGCCAATCCGGATATAG 3’ (Gourse lab) | |
| Sequence-based reagent | TraR_sumo_vector_FOR | IDT, this paper | 5’- AAACATTATGCATAACAAAGCCCGAAAGGAAGCTGAG −3’ (Gourse lab) | |
| Sequence-based reagent | pETsumo_traR_vector_REV | IDT, this paper | 5’- CGGCTTCATCACTTCCACCAATCTGTTCTCTGTGAGCC −3’ (Gourse lab) | |
| Sequence-based reagent | TraR_sumo_fragment_REV | IDT, this paper | 5’- TCGGGCTTTGTTATGCATAATGTTTTCTCTGTCTTTCCTGATACG −3’ (Gourse lab) | |
| Sequence-based reagent | TraR_sumo_fragment_FOR | IDT, this paper | 5’- CAGATTGGTGGAAGTGATGAAGCCGATGAAGCATATTCAG −3’ (Gourse lab) | |
| Sequence-based reagent | rrnBP1(−63 to +20) top | IDT, this paper | 5’- GGTCAGAAAATTATTTTAAATTTCCTCTTGTCA GGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCG −3’ (Darst lab) | |
| Sequence-based reagent | rrnBP1(−63 to +20) bot | IDT, this paper | 5’- CGCCGTTGTTCCGTGTCAGTGGTGGCGCATTAT AGGGAGTTATTCCGGCCTGACAAGAGGAAATTTAAAATAATTTTCTGACC −3’ (Darst lab) | |
| Sequence-based reagent | rpsTP2(−60 to +25) top | IDT, this paper | 5’- GGCGGCGCTTATTTGCACAAATCCATTGACAAA
AGAAGGCTAAAAGGGCATATTCCTCGGCCTTTG
AATTGTCCATATAGAACGC −3’ (Darst lab) |
|
| Sequence-based reagent | rpsTP2 (−60 to +25) bot | IDT, this paper | 5’- GCGTTCTATATGGACAATTCAAAGGCCGAGGAA TAT GCCCTTTTAGCCTTCTTTTGTCAATGGATTTGT GCAAATAAGCGCCGCC −3’ (Darst lab) | |
| Chemical compound, drug | 3-[(3-Cholamidopropyl)dimethylammonio]−2-Hydroxy-1-Propanesulfonate (CHAPSO) | Anatrace | Cat# C317 | |
| Software, algorithm | Bayesian Polishing | PMID: 30412051 | ||
| Software, algorithm | Bsoft | PMID: 23954653 | ||
| Software, algorithm | Coot | PMID: 15572765 | ||
| Software, algorithm | cryoSPARC | PMID: 28165473 | ||
| Software, algorithm | CTFFIND4 | PMID: 26278980 | ||
| Software, algorithm | EMAN2 | PMID: 16859925 | ||
| Software, algorithm | Gautomatch | http://www.mrc-lmb.cam.ac.uk/kzhang/Gautomatch | ||
| Software, algorithm | Gctf | PMID: 26592709 | ||
| Software, algorithm | Molprobity | PMID: 20057044 | ||
| Software, algorithm | MotionCor2 | PMID: 28250466 | ||
| Software, algorithm | Multi-body refinement | PMID: 29856314 | ||
| Software, algorithm | PHENIX | PMID: 20124702 | ||
| Software, algorithm | RELION | PMID: 23000701 | ||
| Software, algorithm | SerialEM | PMID: 16182563 | ||
| Software, algorithm | UCSF Chimera | PMID: 15264254 | ||
| Software, algorithm | Unblur | PMID: 26023829 | ||
| Other | C-flat CF-1.2/1.3 400 mesh gold grids | Electron Microscopy Sciences | Cat# CF413-100-Au |