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. 2019 Oct 25;8:e51230. doi: 10.7554/eLife.51230

Figure 5. Models of the complete HCoV-229E S-protein ectodomain and locations of the N-glycosylation sites.

Figure 5.

(a) A composite model of the complete ectodomain showing the cryo-EM structure determined (enclosed by the box) and a homology model of the HR2 region based on PDB: 2FXP. For each N-glycan observed in the cryo-EM map, the Asn-linked GlcNAc moiety is shown in sphere representation; cyan, those on chain A (orange ribbons). The red dotted circles indicate other potential N-glycosylation sites on chain A. (b) The S2 region of the pre-fusion model. (c) A composite model of the 229E post-fusion form based on the MHV post-fusion structure (PDB: 6B3O) and a crystal structure of the post-fusion 229E HR1-HR2 six-helical bundle (PDB: 5YL9). Red, CH; teal, HR1; orange, HR2. Each potential N-glycan is indicated by a cyan sphere at the Asn sidechain amide group.