Figure 3.

Immunohistochemical staining of Sox10 in the enteric nervous system. A and a are the control group: Sox10 was mostly expressed in glial cells and a few enteric neurons; the nerve plexus and cell morphology were homogeneous. B and b are the intestinal neuronal dysplasia group. The number of glial cells and the area of the nerve plexus markedly proliferated. There were more glial cells than in the control group and giant ganglia in the plexus with more than 8 nerve cells. C and c are the aganglionic segment of HD. No reactivity was seen in ganglia stained with Sox10, but more positively stained nerve fibers and glial cells were observed than the control (A–C: ×200, a–c: ×400). HD, Hirschsprung disease.